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KMID : 0614620080510010019
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Korean Journal of Gastroenterology
2008 Volume.51 No. 1 p.19 ~ p.24
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Sentinel Lymph Node Mapping of the Stomach Using Fluorescent Magnetic Nanoparticles in Rabbits
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Kim Jin-Soo
Yoon Tae-Jong
Kim Hyung-Keun
Kim Sung-Soo
Chae Hiun-Suk
Choi Myung-Gyu
Kim Yong-Jin
Lee Gyu-Chul
Cho Young-Seok
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Abstract
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Background/Aims: Sentinel lymph node (SLN) mapping of the stomach cancer using available techniques is limited by unpredictable lymphatic drainage patterns and skip metastasis. The aim of this study was to test the feasibility of gastric SLN mapping using fluorescent magnetic nanoparticles (FMNP) of uniform nano-size.
Methods: Biocompatible silica-overcoated magnetic nanoparticles containing rhodamine B isothiocyanate (RITC) within a silica shell of controllable thickness with 60 nm thickness were used as model nanomaterials. Gastric lymphatic mapping was performed by injecting 100 μL of either FMNP or isosulafan blue subserosally. Gastric injections (n=7) were made into the body, approximately 5 cm from the lesser curvature of rabbits. Sentinel lymph nodes were visualized using fluorescent nanoparticle detection system.
Results: In 7 rabbits, it was demonstrated that FMNP quickly and accurately detected sentinel lymph nodes. Injection into the stomach resulted in identification of a retrogastric lymph node. Histological analysis in all cases confirmed the presence of nodal tissue.
Conclusions: FMNP can be a potential alternative to existing tracers in the detection of SLN in this animal experiment. (Korean J Gastroenterol 2008;51:19-24)
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KEYWORD
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Sentinel Lymph node, Fluorescent magnetic nanoparticle
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